|
| Status |
Public on Aug 18, 2020 |
| Title |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject HM67 |
| Sample type |
RNA |
| |
|
| Source name |
biopsy from the vastus lateralis muscle, taken from healthy overweight middle-aged male subject, before a 12 week combined exercise training intervention, subject HM67
|
| Organism |
Homo sapiens |
| Characteristics |
subject id: HM67
disease: healthy
time point: before training intervention period
Sex: male
|
| Treatment protocol |
Eighteen healthy middle-aged male subjects performed 12 weeks of combined exercise training. Combined exercise training consisted of 2 endurance exercise sessions and 1 resistance exercise session per week. See Meex et al. (Diabetes. 2010;59(3):572-9. doi: 10.2337/db09-1322 , PMID: 20028948) for more details.
|
| Growth protocol |
Experimental procedures of the 12 week combined exercise training intervention study have been previously published (PMID: 20028948) Eighteen healthy middle-aged male subjects (mean age 59.0 ± 0.8, mean BMI 29.7 ± 0.8 kg/m2; average ± SD) participated in this study.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol and further purified using Rneasy columns. Samples were only included for further analyses in case of intact bands corresponding to 18S and 28S ribosomal subunits and absence of chromosomal peaks or RNA degradation products.
|
| Label |
biotin
|
| Label protocol |
Total RNA (100ng per sample) was labeled with the Whole-Transcript Sense Target Assay (Affymetrix, Santa Clara, CA, USA; P/N 900652).
|
| |
|
| Hybridization protocol |
Hybridization and washing of the Affymetrix GeneChip Human Gene 1.1 ST peg arrays were performed on a GeneTitan Instrument according to the manufacturer's recommendations (Affymetrix, Santa Clara, CA, USA).
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA, USA).
|
| Data processing |
Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.52.1).
|
| |
|
| Submission date |
Aug 14, 2020 |
| Last update date |
Aug 19, 2020 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
[email protected]
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL11532 |
| Series (2) |
| GSE156247 |
Comparative transcriptome analysis of human skeletal muscle in response to cold acclimation and exercise training in human volunteers. [A294] |
| GSE156249 |
Comparative transcriptome analysis of human skeletal muscle in response to cold acclimation and exercise training in human volunteers. |
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