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| Status |
Public on Aug 02, 2021 |
| Title |
Single-oocyte transcriptome analysis reveals aging-associated effects influenced by life stage and calorie restriction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Chromosome segregation errors in oocytes lead to the production of aneuploid eggs, which are the leading cause of pregnancy loss and of several congenital diseases such as Down syndrome. The frequency of chromosome segregation errors in oocytes increases with maternal age, especially at a late stage of reproductive life. How aging at various life stages affects oocytes differently remains poorly understood. In this study, we describe aging-associated changes in the transcriptome profile of mouse oocytes throughout reproductive life. Our single-oocyte comprehensive RNA sequencing using RamDA-seq revealed that oocytes undergo transcriptome changes at a late reproductive stage, whereas their surrounding cumulus cells exhibit transcriptome changes at an earlier stage. Calorie restriction, a paradigm that reportedly prevents aging-associated egg aneuploidy, promotes a transcriptome shift in oocytes with the up-regulation of genes involved in chromosome segregation. This shift is accompanied by the improved maintenance of chromosomal cohesin, the loss of which is a hallmark of oocyte aging and causes chromosome segregation errors. These findings have implications for understanding how oocytes undergo aging-associated functional decline throughout their reproductive life in a context-dependent manner.
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| Overall design |
Single oocytes and their surrounding cumulus cells were analyzed for each age/diet class in two independent experiments: 2 months (Young), 4months, 9 months (Middle), 14 months (Old), 9 months (Calorie Restriction) with hyperovulation, and 2 months without hyperovulation.
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| Contributor(s) |
Mishina T, Tabata N, Hayashi T, Yoshimura M, Umeda M, Mori M, Ikawa Y, Hamada H, Nikaido I, Kitajima TS |
| Citation(s) |
34245092 |
| |
| Submission date |
Oct 09, 2020 |
| Last update date |
Aug 03, 2021 |
| Contact name |
Tomoya S KITAJIMA |
| E-mail(s) |
[email protected]
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| Organization name |
RIKEN
|
| Department |
Center for Biosystems Dynamics Research
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| Lab |
Laboratory for Chromosome Segregation
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| Street address |
2-2-3 Minatojima-minamimachi
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| City |
Kobe |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (401)
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| Relations |
| BioProject |
PRJNA668285 |
| SRA |
SRP286856 |
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