|
| Status |
Public on Oct 29, 2020 |
| Title |
RNAseq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptome analysis plays a central role in elucidating the complexity of gene expression regulation in Escherichia coli. By analyzing the transcriptomics of E. coli treated with water, acetone, and Cinnamomum camphora essential oil, the inhibitory mechanism of the essential oil on the human intestinal microbe was studied. The results showed that the inhibitory effect of the essential oil on E. coli increased with an increase in concentration; 1/4 minimum inhibitory concentration was the reaction equilibrium point. RNAseq transcriptomic comparison indicated that the essential oil inhibited the growth of E. coli by inhibiting the metabolism, chemotaxis, and some resistance reactions of them, while E. coli maintained its life activities by enhancing its resistance reactions. These results are of great importance to the study of the medical use of C. camphor essential oil and gene regulation in E. coli under stress conditions.
|
| |
|
| Overall design |
Transcriptome sequencing of RNA under five different treatments in E. coli
|
| |
|
| Contributor(s) |
Yu Y, Dong J, Wang Y, Gong X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 28, 2020 |
| Last update date |
Oct 29, 2020 |
| Contact name |
tian Yu |
| E-mail(s) |
[email protected]
|
| Phone |
18379993723
|
| Organization name |
Human Aging Research Institute and School of Life Science, Nanchang University, and Jiangxi Key Laboratory of Human Aging
|
| Street address |
999 Xuefu Rd.
|
| City |
Nanchang |
| ZIP/Postal code |
330031 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL25368 |
Illumina NovaSeq 6000 (Escherichia coli) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA672882 |
| SRA |
SRP289443 |
Less...
More...