 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 04, 2021 |
| Title |
Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Adaptive plasticity in stress responses is a key element of plant survival strategies. For instance, moderate heat stress (HS) primes a plant to acquire thermotolerance, which allows subsequent survival of more severe HS conditions. Acquired thermotolerance is actively maintained over several days (HS memory) and involves the sustained induction of memory-related genes. We find FORGETTER3/ HEAT SHOCK TRANSCRIPTION FACTOR A3 (FGT3/HSFA3) to be specifically required for physiological HS memory and maintaining high memory-gene expression during the days following a HS exposure. HSFA3 mediates HS memory by direct transcriptional activation of memory-related genes after return to normal growth temperatures. HSFA3 binds HSFA2, and in vivo both proteins form heteromeric complexes with additional HSFs. Our results indicate that only complexes containing both HSFA2 and HSFA3 efficiently promote transcriptional memory by promoting histone H3 lysine 4 (H3K4) hyper-methylation. In summary, our work defines the major HSF complex controlling transcriptional memory and elucidates the in vivo dynamics of HSF complexes during somatic stress memory.
|
| |
|
| Overall design |
Gene expression in wild type, hsfa2 and hsfa3 single mutants and hsfa2 hsfa3 double mutants for non treated controls and for plants 4, 28 and 52 hours after acclimatation. Three biological replicates for each combination.
|
| |
|
| Contributor(s) |
Friedrich T, Oberkofler V, Kappel C, Bäurle I |
| Citation(s) |
34103516 |
| |
| Submission date |
Dec 01, 2020 |
| Last update date |
Jul 08, 2021 |
| Contact name |
Isabel Bäurle |
| E-mail(s) |
[email protected]
|
| Phone |
+49 331 9772647
|
| Organization name |
Universität Potsdam
|
| Department |
Institut für Biochemie und Biologie
|
| Street address |
Karl-Liebknecht-Str. 24-25
|
| City |
Potsdam |
| ZIP/Postal code |
14476 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24025 |
BGISEQ-500 (Arabidopsis thaliana) |
|
| Samples (48)
|
| GSM4951976 |
1-Col-NTC |
| GSM4951977 |
1-hsfa2,3-ACC+28h |
| GSM4951978 |
1-hsfa2,3-ACC+4h |
| GSM4951979 |
1-hsfa2,3-ACC+52h |
| GSM4951980 |
1-hsfa2,3-NTC |
| GSM4951981 |
1-hsfa2-ACC+28h |
| GSM4951982 |
1-hsfa2-ACC+4h |
| GSM4951983 |
1-hsfa2-ACC+52h |
| GSM4951984 |
1-hsfa2-NTC |
| GSM4951985 |
1-hsfa3-ACC+28h |
| GSM4951986 |
1-hsfa3-ACC+4h |
| GSM4951987 |
1-hsfa3-ACC+52h |
| GSM4951988 |
1-hsfa3-NTC |
| GSM4951989 |
2-Col-ACC+28h |
| GSM4951990 |
2-Col-ACC+4h |
| GSM4951991 |
2-Col-ACC+52h |
| GSM4951992 |
2-Col-NTC |
| GSM4951993 |
2-hsfa2,3-ACC+28h |
| GSM4951994 |
2-hsfa2,3-ACC+4h |
| GSM4951995 |
2-hsfa2,3-ACC+52h |
| GSM4951996 |
2-hsfa2,3-NTC |
| GSM4951997 |
2-hsfa2-ACC+28h |
| GSM4951998 |
2-hsfa2-ACC+4h |
| GSM4951999 |
2-hsfa2-ACC+52h |
| GSM4952000 |
2-hsfa2-NTC |
| GSM4952001 |
2-hsfa3-ACC+28h |
| GSM4952002 |
2-hsfa3-ACC+4h |
| GSM4952003 |
2-hsfa3-ACC+52h |
| GSM4952004 |
2-hsfa3-NTC |
| GSM4952005 |
3-Col-ACC+28h |
| GSM4952006 |
3-Col-ACC+4h |
| GSM4952007 |
3-Col-ACC+52h |
| GSM4952008 |
3-Col-NTC |
| GSM4952009 |
3-hsfa2,3-ACC+28h |
| GSM4952010 |
3-hsfa2,3-ACC+4h |
| GSM4952011 |
3-hsfa2,3-ACC+52h |
| GSM4952012 |
3-hsfa2,3-NTC |
| GSM4952013 |
3-hsfa2-ACC+28h |
| GSM4952014 |
3-hsfa2-ACC+4h |
| GSM4952015 |
3-hsfa2-ACC+52h |
| GSM4952016 |
3-hsfa2-NTC |
| GSM4952017 |
3-hsfa3-ACC+28h |
| GSM4952018 |
3-hsfa3-ACC+4h |
| GSM4952019 |
3-hsfa3-ACC+52h |
| GSM4952020 |
3-hsfa3-NTC |
|
| Relations |
| BioProject |
PRJNA681862 |
| SRA |
SRP295378 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162434_RAW.tar |
6.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...