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| Status |
Public on Apr 11, 2024 |
| Title |
Single cell RNA sequencing profile of unique mesenchymal progenitor subsets from the murine brain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The sensitive central nervous system is protected from potentially toxic components in the circulation by a semi-permeable interface, the blood brain barrier (BBB). This barrier is tightly regulated by several stromal cell types, however in certain regions of the brain it requires distinct properties. In particular, two of these regions, the choroid plexus and the pituitary gland are considered differentially permeable compared to other regions. Perivascular mesenchymal progenitors are intimately associated with endothelium and are therefore key participants in the regulation of the BBB. Using a mesenchymal progenitor specific lineage tracing tool, gene expression in this population from the choroid plexus and pituitary gland was profiled at the single cell level to investigate the genetic programs that allow these regions to maintain their respective specific pervious properties.
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| Overall design |
Single cell RNA-seq analyes of mesenchymal progenitors from the choroid plexus and pituitary gland. A lower proportion of other nucleated cell types were included for comparison.
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| Contributor(s) |
Bastedo W, Scott RW, Underhill TM |
| Citation(s) |
38575991 |
| |
| Submission date |
Dec 06, 2020 |
| Last update date |
Apr 12, 2024 |
| Contact name |
Tully Michael Underhill |
| E-mail(s) |
[email protected]
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| Phone |
604-822-5833
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| Organization name |
University of British Columbia
|
| Department |
Biomedical Research Centre
|
| Street address |
2222 Health Sciences Mall
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T 1Z3 |
| Country |
Canada |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
| GSM4958649 |
Single cell RNA-seq - adult tdTomato MPs enriched choroid plexus |
| GSM4958650 |
Single cell RNA-seq - adult tdTomato MPs enriched pituitary gland |
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| Relations |
| BioProject |
PRJNA682804 |
| SRA |
SRP296555 |
