|
| Status |
Public on Apr 11, 2024 |
| Title |
Single cell RNA-seq - adult tdTomato MPs enriched pituitary gland |
| Sample type |
SRA |
| |
|
| Source name |
Quiescent Hic1CreERT2/CreERT2;Rosa26LSLtdTomato/tdTomato MP enriched pituitary gland
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: pituitary gland
cell type: Mesenchymal Progenitors
genotype: Hic1CreERT2/CreERT2;Rosa26LSLtdTomato
|
| Treatment protocol |
Adult animals 8 weeks of age were injected with 2.5mg tamoxifen in 100uL sunflower oil daily for 5 consecutive days and allowed a ten day washout period before experments were performed.
|
| Growth protocol |
Mice were bred and raised to 8 weeks of age using standard approved animal care committee methodology.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
FACS purified cells were counted and inspected by hemocytometer before input to the 10X chromium library preparation pipeline and subsequent NGS.
10X Chromium Single Cell 3' v2 or v3
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Samples were demultiplexed using cellranger mkfastq with bclfastq2.
Samples were aligned and output matrices generated using cellranger count for alignment.
cellranger aggregate was used to aggregate multiple samples for combined analysis
Genome_build: mm10 with appended tdTomato and EGFP sequences
Supplementary_files_format_and_content: gene matrices, cell barcode and gene list are included as .mtx, .tsv and .tsv files, respectively
|
| |
|
| Submission date |
Dec 06, 2020 |
| Last update date |
Apr 11, 2024 |
| Contact name |
Tully Michael Underhill |
| E-mail(s) |
[email protected]
|
| Phone |
604-822-5833
|
| Organization name |
University of British Columbia
|
| Department |
Biomedical Research Centre
|
| Street address |
2222 Health Sciences Mall
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T 1Z3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE162733 |
Single cell RNA sequencing profile of unique mesenchymal progenitor subsets from the murine brain |
|
| Relations |
| BioSample |
SAMN17010884 |
| SRA |
SRX9637224 |
