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| Status |
Public on Dec 14, 2020 |
| Title |
Expression data from APC 50% or 80% -treated HFDPCs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling reveals a potential role of APC 50% or APC 80% in stimulating hair growth in dermal papilla cells.
HFDPCs were human primary cells line, treated with 5 μg/ml APC 50% for 48 h. Microarray gene expression profiling was conducted for three biological replicates
HFDPCs were human primary cells line, treated with 5 μg/ml APC 80% for 48 h. Microarray gene expression profiling was conducted for three biological replicates
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| Overall design |
RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis with GeneChip® 3' Expression Arrays using 3' IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). Two hundred and fifty ng of total RNA from each sample was used to generate amplified and biotinylated complementary RNA (cRNA) from poly (A) RNA in a total RNA sample according to the user manual. IVT Incubation time was 16 hour. GeneAtlas® Hybridization, Wash and Stain Kit was used for hybridizing 3' IVT Array Strips according to the user manual (P/N 08-0306). Human genome array strips (HG-U219) were hybridized for 16 hours in a 45oC incubator, washed and stained and finally imaging was done with the GeneAtlas Fluidics and Imaging Station.
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| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 13, 2020 |
| Last update date |
Dec 18, 2020 |
| Contact name |
Meriem Bejaoui |
| E-mail(s) |
[email protected]
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| Organization name |
University of Tsukuba
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| Street address |
1 Chome-1-1 Tennodai
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| City |
Tsukuba, |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8577 |
| Country |
Japan |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA684890 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE163091_RAW.tar |
19.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| GSE163091_normalized_data_and_statistics_APC50.txt.gz |
2.6 Mb |
(ftp)(http) |
TXT |
| GSE163091_normalized_data_and_statistics_APC80.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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