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| Status |
Public on Mar 01, 2010 |
| Title |
Expression data from placenta harvested from WT and Pth-null fetuses treated 90 minutes prior with saline or PTH (1-84) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Parathyroid hormone (PTH) plays an essential role in regulating calcium and bone homeostasis in the adult, but whether PTH is required at all for regulating fetal-placental mineral homeostasis is uncertain. To address this we treated Pth-null mice in utero with 1 nmol PTH (1-84) or saline and examined placental calcium transfer 90 minutes later. It was found that placental calcium transfer increased in Pth-null fetuses treated with PTH as compared to Pth-null fetuses treated with saline. Subsequently, to determine the effect of PTH treatment on placental gene expression, in a separate experiment, 90 minutes after the fetal injections the placentas were removed for subsequent RNA extraction and microarray analysis.
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| Overall design |
12 mouse placentas were selected for RNA extraction and hybridization on the Affymetrix Mouse Gene 1.0 ST Array. Of the 12 placenta, 3 were from WT fetuses treated in utero with saline, 3 were from WT fetuses treated in utero with PTH (1-84), 3 were from Pth-null fetuses treated in utero with saline, and 3 were from Pth-null fetuses treated in utero with PTH (1-84).
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| Contributor(s) |
Simmonds CS, Kovacs CS |
| Citation(s) |
19968565 |
| |
| Submission date |
Jul 07, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Christopher S Kovacs |
| E-mail(s) |
[email protected]
|
| Phone |
1-709-777-6881
|
| Fax |
1-709-777-8049
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| URL |
http://www.med.mun.ca/Medicine/Faculty/Kovacs.aspx
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| Organization name |
Memorial University of Newfoundland
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| Department |
Faculty of Medicine - Endocrinology
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| Lab |
Calcium Research Laboratory
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| Street address |
Health Sciences Centre, 300 Prince Philip Drive
|
| City |
St. John's |
| State/province |
Newfoundland |
| ZIP/Postal code |
A1B 3V6 |
| Country |
Canada |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA117287 |
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