|
| Status |
Public on Feb 21, 2010 |
| Title |
12h pathway stimulation / inhibition experiment in epiblast stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment records the transcriptional responses of EpiSCs (line E3) to FGF/ERK inhibition, GSK3ß inhibition, LIF/STAT3 activation, as well as combined treatment for 12 hours each.
|
| |
|
| Overall design |
EpiSCs were grown in KSR medium on low-density feeders. At semiconfluency, media were changed as detailed for the individual samples. Cells were harvested for RNA isolation 12 hours later.
|
| |
|
| Citation(s) |
20207225 |
| |
| Submission date |
Jul 16, 2009 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Boris Greber |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (5)
|
|
| This SubSeries is part of SuperSeries: |
| GSE17202 |
Expression profiles of epiblast stem cells and embryonic stem cells |
|
| Relations |
| BioProject |
PRJNA123653 |
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