|
| Status |
Public on Feb 21, 2010 |
| Title |
12h PCL-treated EpiSCs |
| Sample type |
RNA |
| |
|
| Source name |
cell culture from E3 epiblast stem cells
|
| Organism |
Mus musculus |
| Characteristics |
cell line: E3
|
| Treatment protocol |
see below
|
| Growth protocol |
Grown on inactivated mouse embryonic fibroblasts (CF1) in KSR medium supplemented with 5 ng/ml FGF2. At semiconfluence change to KSR medium with 1uM PD0325901 / 3uM CHIR99021 / 1000 U/ml LIF for 12h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit with on-column DNA digestion
|
| Label |
Cy-3
|
| Label protocol |
Ambion TotalPrep RNA Amplification Kit. RNA in put: 400ng, IVT: 12h
|
| |
|
| Hybridization protocol |
According to manufacturer's instructions. Hybridization time: 18h
|
| Scan protocol |
Illumina iScan system using default settings
|
| Description |
N/A
|
| Data processing |
Cubic Spline normalization with background subtraction in BeadStudio. Values below 50% of the detection threshold intensity (detection p value = 0.010) were set to "Null" (= not expressed / detectable).
|
| |
|
| Submission date |
Jul 16, 2009 |
| Last update date |
Feb 20, 2010 |
| Contact name |
Boris Greber |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE17134 |
12h pathway stimulation / inhibition experiment in epiblast stem cells |
| GSE17202 |
Expression profiles of epiblast stem cells and embryonic stem cells |
|
