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| Status |
Public on Jul 16, 2010 |
| Title |
Gene expression profiles of adult visceral epithelium (syn: podocyte layer) isolated from MafB-GFP transgenic mice using FACS. (GUDMAP Series ID: 31) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone.
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| Overall design |
Mafb-EGFP BAC transgenic mice were utilized to isolate the podocyte cells from the glomerlus from adult kidneys. The podocyte cells were isolated from the kidney using a combination of Collagenase A digestion, sieving and tyrpsinization. RNA was isolated and the gene expression profiles were determined by microarrays.
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| Web link |
http://www.gudmap.org
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| Contributor(s) |
Potter S, Brunskill E |
| Citation(s) |
21931791 |
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| Submission date |
Jul 16, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
GUDMAP Developers |
| E-mail(s) |
[email protected]
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| Phone |
+44 131 651 8500
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| Organization name |
IGMM MRC Human Genetics Unit
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| Lab |
GUDMAP Database Group
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| Street address |
Crewe Road
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| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE17218 |
Encyclopedia of the expression levels of all genes in multiple components of the developing kidney |
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| Relations |
| BioProject |
PRJNA123643 |
