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| Status |
Public on Sep 15, 2021 |
| Title |
Human blastoids model blastocyst development and implantation [scRNAseq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Shortly after fertilization, human embryos implant into the uterus. This requires the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form blastocyst models, which we termed blastoid. Here we show that naïve human pluripotent stem cells (hPSCs) triply inhibited for the Hippo, TGFb, and ERK pathways consistently and efficiently (>70%) form blastoids that generate transcriptional pre-implantation (>95%) analogs of the three founding lineages (trophoblast, epiblast, hypoblast), and in the sequential and timely manner of blastocysts. Blastoids spontaneously form an axis marked by maturation of the polar region, which acquires the potential to specifically attach to hormonally-stimulated endometrial cells, as during in utero implantation. Such human blastoids are scalable, versatile, and ethical models to explore human implantation and development.
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| |
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| Overall design |
Blastoids and stem cells with various treatments
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| |
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| Contributor(s) |
Kagawa H, Javali A, Khoei HH, Sommer TM, Reimer YS, Sestini G, Rivron N |
| Citation(s) |
34856602 |
| |
| Submission date |
Jun 12, 2021 |
| Last update date |
Dec 15, 2021 |
| Contact name |
Giovanni Sestini |
| Organization name |
IMBA - Institute of Molecular Biotechnology
|
| Street address |
Dr.-Bohr-Gasse 3, 1030 Wien
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (2715)
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| This SubSeries is part of SuperSeries: |
| GSE177689 |
Human blastoids model blastocyst development and implantation |
|
| Relations |
| BioProject |
PRJNA737134 |
| SRA |
SRP323840 |
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