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| Status |
Public on Sep 15, 2021 |
| Title |
Human blastoids model blastocyst development and implantation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
About one week after fertilization, human embryos implant into the uterus. This necessitates the formation of a blastocyst consisting of a sphere encircling a cavity lodging the embryo proper. Stem cells can form a blastocyst model, which we termed blastoid. Here we show that naive human pluripotent stem cells (PXGL hPSCs) efficiently (>70%) form blastoids generating blastocyst-stage analogs of the 3 founding lineages (>97% trophectoderm, epiblast, and primitive endoderm) according to the sequence and to the pace of blastocyst development. Blastoids form the first axis and we observe that the epiblast induces the maturation of the polar trophectoderm that consequently acquires the specific and transient potential to attach to hormonally-stimulated endometrial cells. Such human blastoids are faithful, scalable, versatile, and ethical models to explore human implantation and development.
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| |
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| Overall design |
Refer to individual Series
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| |
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| Citation(s) |
34856602 |
| |
| Submission date |
Jun 12, 2021 |
| Last update date |
Dec 15, 2021 |
| Contact name |
Giovanni Sestini |
| Organization name |
IMBA - Institute of Molecular Biotechnology
|
| Street address |
Dr.-Bohr-Gasse 3, 1030 Wien
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2776)
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| This SuperSeries is composed of the following SubSeries: |
| GSE177616 |
Human blastoids model blastocyst development and implantation [scRNAseq] |
| GSE179040 |
Human blastoids model blastocyst development and implantation [Bulk RNA-seq] |
|
| Relations |
| BioProject |
PRJNA737139 |
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