|
| Status |
Public on Nov 30, 2021 |
| Title |
Transcription profiling of MamBo cell lines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We studied cell lines derived from two transgenic mammary tumors driven by human HER2 that showed different dynamics of HER2 status. MamBo89 (HER2 stable) cell line displayed high and stable HER2 expression, which was maintained upon in vivo passages, whereas MamBo43 (HER2 labile) cell line gave rise to HER2-negative tumors, from which MamBo38 (HER2 loss) cell line was derived. MamBo cell lines were established from mammary tumors of FVBhuHER2 virgin female mice.
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| |
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| Overall design |
Transcription analysis was performed on the three cell lines with and without treatment with trastuzumab.
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| Contributor(s) |
Giusti V, Ruzzi F, Landuzzi L, Ianzano ML, Laranga R, Nironi E, Scalambra L, Nicoletti G, De Giovanni C, Olivero M, Arigoni M, Calogero R, Nanni P, Palladini A, Lollini P |
| Citation(s) |
34775465 |
| |
| Submission date |
Aug 04, 2021 |
| Last update date |
Nov 30, 2021 |
| Contact name |
Raffaele A Calogero |
| E-mail(s) |
[email protected]
|
| Phone |
++39 0116706454
|
| Organization name |
University of Torino
|
| Department |
Molecular Biotechnology Center
|
| Lab |
Bioinformatics and Genomics Unit
|
| Street address |
Via Nizza 52
|
| City |
Torino |
| State/province |
To |
| ZIP/Postal code |
10126 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (11)
|
| GSM5503019 |
MAMBO38TRT190617 Trastuzumab 30 µg/ml 60 days |
| GSM5503020 |
MAMBO43NT241016 Untreated |
| GSM5503021 |
MAMBO43TRNT241016 Trastuzumab 30 µg/ml 30 days then w/o 30 days |
| GSM5503022 |
MAMBO43TRT241016 Trastuzumab 30 µg/ml 60 days |
| GSM5503023 |
MAMBO89AG3241016 Untreated |
| GSM5503024 |
MAMBO89NT241016 Untreated |
| GSM5503025 |
MAMBO89TRNT241016 Trastuzumab 30 µg/ml 30 days then w/o 30 days |
| GSM5503026 |
MAMBO89TRT241016 Trastuzumab 30 µg/ml 60 days |
|
| Relations |
| BioProject |
PRJNA752119 |
| SRA |
SRP331152 |
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