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| Status |
Public on Mar 29, 2022 |
| Title |
Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Human doublecortin (DCX) mutations are associated with severe brain malformations leading to aberrant neuron positioning (heterotopia), intellectual disability and epilepsy. DCX is a microtubule-associated protein which plays a key role during neurodevelopment in neuronal migration and differentiation. Dcx knockout (KO) mice show disorganized hippocampal pyramidal neurons. The CA2/CA3 pyramidal cell layer is present as two abnormal layers and disorganized CA3 KO pyramidal neurons are also more excitable than wild-type (WT) cells. To further identify abnormalities, we characterized Dcx KO hippocampal neurons at subcellular, molecular and ultrastructural levels. Severe defects were observed in mitochondria, affecting number and distribution. Also, the Golgi apparatus was visibly abnormal, increased in volume and abnormally organized. Transcriptome analyses from laser microdissected hippocampal tissue at postnatal day 60 (P60) highlighted organelle abnormalities. Ultrastructural studies of CA3 cells performed in P60 (young adult) and > 9 months (mature) tissue showed that organelle defects are persistent throughout life. Locomotor activity and fear conditioning behavior of young and mature adults was also abnormal: KO mice consistently performed less well than WT littermates, with defects becoming more severe with age. Thus, we show that disruption of a neurodevelopmentally-regulated gene can lead to permanent organelle anomalies contributing to abnormal adult behavior
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| Overall design |
14 samples analyzed that included 5 WT and 9 mutant doublecortin (Dcx). In WT animals, the hippocampal CA3 region was microdissected in one piece. In Dcx KO animals, internal stratum pyramidale (SPI) and external stratum pyramidale (SPE) CA3 cells in the two layers were separated.
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| Contributor(s) |
Stouffer M, Khalaf-Nazzal R, Diaz-Cifuentes C, Albertini G, Bandet E, Grannec G, Lavilla V, Deleuze J, Olaso R, Nosten-Bertrand M, Francis F |
| Citation missing |
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| Submission date |
Sep 29, 2021 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Robert Olaso |
| E-mail(s) |
[email protected]
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| Phone |
33(1)601632112
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| Organization name |
CEA-DRF
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| Department |
CNRGH
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| Lab |
L2PGH
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| Street address |
2 rue Gaston Cremieux
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| City |
Evry Cedex |
| ZIP/Postal code |
91057 |
| Country |
France |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA767411 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE185031_Non-normalized_data.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
| GSE185031_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
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