|
| Status |
Public on Mar 29, 2022 |
| Title |
166-1 inner |
| Sample type |
RNA |
| |
|
| Source name |
Dcx N°166, internal stratum pyramidale (SPI) CA3 cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Dcx
background strain: Sv129Pas
developmental stage: Adult
tissue: Brain
brain region: internal stratum pyramidale (SPI) CA3 cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from pooled microdissected material using an RNA isolation kit (Qiagen RNAeasy microkit) according to manufacturer’s instructions. RNA quantity was measured using a nanodrop spectrophotometer. RNA quality was checked using an Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip Kit (5065-4473, Agilent Technologies, Palo Alto) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 12
|
| Data processing |
The data were normalised using quantile normalisation with Beadstudio
|
| |
|
| Submission date |
Sep 29, 2021 |
| Last update date |
Mar 29, 2022 |
| Contact name |
Robert Olaso |
| E-mail(s) |
[email protected]
|
| Phone |
33(1)601632112
|
| Organization name |
CEA-DRF
|
| Department |
CNRGH
|
| Lab |
L2PGH
|
| Street address |
2 rue Gaston Cremieux
|
| City |
Evry Cedex |
| ZIP/Postal code |
91057 |
| Country |
France |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE185031 |
Doublecortin mutation leads to persistent defects in the Golgi apparatus and mitochondria in adult hippocampal pyramidal cells |
|
