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| Status |
Public on Nov 20, 2021 |
| Title |
RNA-sequencing results of five samples across three YMC cycles of both wt and yaf9W89A strains |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of the experiments is to study how the absence of Yaf9-H3 acyl reading affected metabolic and global gene transcription. We performed RNA-seq for our wt and yaf9W89A cells across three consecutive YMC cycles. We found that HOC genes maintained expression in the LOC phase and that LOC genes were not properly expressed in the LOC phase.
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| Overall design |
5 time points of YMC samples were collected across three consecutive cycles for both wt and yaf9W89A strains. Samples WT1-WT5 cover one wt YMC cycle. WT6-WT10 and WT11-WT15 are replicates; Samples W89A16-W89A20 cover one yaf9W89A YMC cycle. W89A21-W89A25 and W89A26-W89A30 are replicates. The triplicates were processed with RNA sequencing technology.
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| Citation(s) |
34819351 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM126900 |
Mechanisms of chromatin and transcriptional regulation |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
Brian D Strahl |
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| |
| Submission date |
Nov 18, 2021 |
| Last update date |
Feb 21, 2022 |
| Contact name |
Brian David Strahl |
| E-mail(s) |
[email protected]
|
| Phone |
9198433896
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Biochemistry
|
| Street address |
120 Mason Farm Road 3060 Genetic Medicine
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA781537 |
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