|
| Status |
Public on Mar 31, 2010 |
| Title |
Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To determine the effect of Sox17 overexpression in mouse embryonic stem (ES) cells, we performed gain-of-function analysis by generating ES cell lines carrying a doxycycline inducible FLAG-tagged Sox17 transgene. We treated Sox17-inducible ES cells with doxycycline, collected RNA and performed genome-wide transcriptional analysis. We found that genes invovled in adhesion function and basement membrane establishment were transcriptionally upregulated in ES cells upon induction of Sox17. We also observed downregulation in the transcription of genes involved in pathways known to be functionally important for ES cell pluripotency and self-renewal. However, Sox17 expression was not sufficient to rapidly down-regulate Sox2, Nanog, and Oct4.
|
| |
|
| Overall design |
Two independent doxycycline inducible Sox17-overexpressing mouse embryonic stem cells were derived. The genes expression changes in the Sox17-induced cells were compared to untreated (no doxycycline) controls and to control cells treated with or without doxycycline. The total RNA from these samples were amplified using Ambion Illumina TotalPrep RNA Amplification kit and arrayed on Illumina MouseRef8 v2 chips.
|
| |
|
| Contributor(s) |
Niakan KK, Sherwood RI |
| Citation(s) |
20123909 |
| |
| Submission date |
Nov 13, 2009 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Kathy Niakan |
| Organization name |
Harvard University
|
| Department |
Stem Cell and Regenerative Biology
|
| Lab |
Kevin Eggan
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (6)
|
| GSM470907 |
Control KH2 cells treated with doxycycline |
| GSM470908 |
Control untreated KH2 cells |
| GSM470909 |
Sox17-inducible ES cells treated with doxycycline |
| GSM470910 |
Untreated Sox17-inducible ES cells |
| GSM470911 |
Sox17-inducible-line 2 ES cells treated with doxycyline |
| GSM470912 |
Untreated Sox17-inducible-line 2 ES cells |
|
| Relations |
| BioProject |
PRJNA120625 |
Less...
More...