|
| Status |
Public on Mar 31, 2010 |
| Title |
Sox17-inducible ES cells treated with doxycycline |
| Sample type |
RNA |
| |
|
| Source name |
Sox17-inducible ES cells treated with doxycycline
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Mouse embryonic stem cell
|
| Treatment protocol |
Sox17-inducible ES cells were treated with 1μg/mL of doxycycline for 5days prior to harvesting.
|
| Growth protocol |
Sox17-inducible ES cells were grown for 5days prior to harvesting. ES media consists of: 85% KO-DMEM; 15% fetal bovine serum (Hyclone); 1% L-glutamine (Invitrogen); 1% β-mercaptoethanol (Invitrogen); 1% Penicillin/Streptomycin (Invitrogen) 103IU of LIF (Chemicon). No MEFs were used in these experiments.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kit (Ambion)
|
| |
|
| Hybridization protocol |
Illumina BeadStation (Illumina)
|
| Scan protocol |
Illumina BeadStation (Illumina)
|
| Description |
Sox17-inducible mouse embryonic stem cell
4592728087_C
|
| Data processing |
Illumina BeadStudio (Illumina); rank invariant normalization, background subtraction using Illumina BeadStudio (Illumina)
|
| |
|
| Submission date |
Nov 13, 2009 |
| Last update date |
Nov 17, 2009 |
| Contact name |
Kathy Niakan |
| Organization name |
Harvard University
|
| Department |
Stem Cell and Regenerative Biology
|
| Lab |
Kevin Eggan
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE19028 |
Gene Expression Analysis of Sox17-induced Mouse Embryonic Stem Cells |
|
