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| Status |
Public on Dec 11, 2009 |
| Title |
Transgenic rice line carrying the maize resistance gene Rxo1 to Xanthomonas oryzae pv. oryzicola |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
Genome-wide gene responses in a transgenic rice line carrying the maize resistance gene Rxo1 to the rice bacterial streak pathogen, Xanthomonas oryzae pv. oryzicola
Non-host resistance in rice to its bacterial pathogen, Xanthomonas oryzae pv. oryzicola (Xoc), mediated by a maize NBS-LRR type R gene, Rxo1 shows a typical hypersensitive reaction (HR) phenotype, but the molecular mechanism(s) underlying this type of non-host resistance remain largely unknown. Results A microarray experiment was performed to reveal the molecular mechanisms underlying HR of rice to Xoc mediated by Rxo1 using a pair of transgenic and non-transgenic rice lines. Our results indicated that Rxo1 appeared to function in the very early step of the interaction between rice and Xoc, and could specifically activate large numbers of genes involved in signaling pathways leading to HR and some basal defensive pathways such as SA and ET pathways. In the former case, Rxo1 appeared to differ from the typical host R genes in that it could lead to HR without activating NDR1. In the latter cases, Rxo1 was able to induce a unique group of WRKY TF genes and a large set of genes encoding PPR and RRM proteins that share the same G-box in their promoter regions with possible functions in post-transcriptional regulation.
In conclusion, Rxo1, like most host R genes, was able to trigger HR against Xoc in the heterologous rice plants by activating multiple defensive pathways related to HR, providing useful information on the evolution of plant resistance genes. Maize non-host resistance gene Rxo1 could trigger the pathogen-specific HR in heterologous rice, and ultimately leading to a localized programmed cell death which exhibits the characteristics consistent with those mediated by host resistance genes, but a number of genes encoding pentatricopeptide repeat and RNA recognition motif protein were found specifically up-regulated in the Rxo1 mediated disease resistance. These results add to our understanding the evolution of plant resistance genes.
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| Overall design |
All inoculated flag leaves from three plants of 9804-Rxo1 and 9804 were collected two days post-inoculation (dpi), and the flag leaves from three non-inoculated check plants of 9804-Rxo1 and 9804 were also sampled at the same time point as control. The flag leaves from non-inoculated check plants of 9804 were sampled at the same time point as control: 9804-Rxo1 (3-replications), XOC - time: 2 dpi(3-replications); 9804-Rxo1 (3-replications); 9804, XOC - time: 2 dpi(3-replications) 9804. 2 sets of separately normalized data: 9804-Rxo1 and 9804.
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| Contributor(s) |
Zhou Y, Xu M, Zhao M, Xie X, Zhu L, Fu B, Li Z |
| Citation(s) |
20122142 |
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| Submission date |
Dec 01, 2009 |
| Last update date |
Dec 27, 2017 |
| Contact name |
Meirong Xu |
| Organization name |
Chinese academy of agricultural sciences
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| Department |
Institute of Crop Sciences
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| Lab |
rice molecular genetics and molecular breeding
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| Street address |
12 South Zhong-Guan-Cun St.
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
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| Samples (12)
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| GSM476769 |
Rice-9804-Rxo1-Xoc, biological rep1 |
| GSM476770 |
Rice-9804-Rxo1-Xoc, biological rep2 |
| GSM476771 |
Rice-9804-Rxo1-Xoc, biological rep3 |
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| Relations |
| BioProject |
PRJNA120769 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19239_RAW.tar |
53.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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