|
| Status |
Public on Dec 11, 2009 |
| Title |
Rice-9804-Rxo1, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
9804 carrying the maize resistance gene Rxo1, leaf, CK
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: leaf
genotype: Japonica, 9804 carrying the maize resistance gene Rxo1
developmental stage: booting stage
|
| Treatment protocol |
All inoculated flag leaves from three plants of 9804-Rxo1 and 9804 were collected two days post-inoculation (dpi), and the flag leaves from three non-inoculated check plants of 9804-Rxo1 and 9804 were also sampled at the same time point as control. The collected leaves were immediately frozen in liquid nitrogen, and then kept at -70°C. The flag leaves from non-inoculated check plants of 9804 were sampled at the same time point as control. The collected leaves were immediately frozen in liquid nitrogen, and then kept at -70°C.
|
| Growth protocol |
Seeds were sown in the seedling nursery, and ten of 30-days-old seedlings of each line were transplanted into an isolated screening house at a spacing of 25×20 cm with 1 seedling per hill in the Fujian Academy of Agricultural Sciences.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted from each frozen leaf sample using the TRIZOL reagent according to the instruction, and then purified and concentrated using the Qiagen RNeasy MinElute Cleanup kit. RNA was quality-checked and quantified using a Bioanalyzer 2100 (Agilent Technologies, Cheadle, UK).
|
| Label |
biotin
|
| Label protocol |
Biotin labeled cRNA was produced with 5 ug of total RNA as starting material for each sample reaction.
|
| |
|
| Hybridization protocol |
RNA from the three independent replicate samples of each treatment were used for hybridization to the Affymetrix Genechips® Rice Genome Array chips, which contain probes to query 51,279 transcripts from two rice cultivars, including 48,564 japonica transcripts and 1,260 indica transcripts from Affymetrix (Santa Clara, CA).Hybridization to the arrays and quality control checks were carried out at the Company of CapitalBio Corporation, Beijing. (Affymetrix GeneChip Expression Analysis g of this fragmented target cRNA wasmTechnical Manual).
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
A homozygous T4 line containing a single copy of Rxo1 from transgenic 9804 plants (the standard Agrobacterium mediated transformation system)
|
| Data processing |
MaAS 5.0
|
| |
|
| Submission date |
Dec 01, 2009 |
| Last update date |
Dec 11, 2009 |
| Contact name |
Meirong Xu |
| Organization name |
Chinese academy of agricultural sciences
|
| Department |
Institute of Crop Sciences
|
| Lab |
rice molecular genetics and molecular breeding
|
| Street address |
12 South Zhong-Guan-Cun St.
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE19239 |
Transgenic rice line carrying the maize resistance gene Rxo1 to Xanthomonas oryzae pv. oryzicola |
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