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| Status |
Public on Jul 19, 2010 |
| Title |
Expression Data from BALB/c and Stat6-deficient bone marrow derived macrophages (BMDM) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We used microarrays to find Stat6 dependent genes in control and IL-4 exposed bone marrow derived macrophages.
Alternatively activated macrophages (AAM) accumulate in tissues during Th2-associated immune responses like helminth infections and allergic disorders. These cells possess potent inhibitory activity against T cells. The differentiation of AAM depends on IL-4/IL-13-mediated activation of the transcription factor Stat6. Stat6 is also required in AAM to induce several genes, such as YM1, FIZZ1 and Arginase1.
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| Overall design |
Macrophages were differentiated from bone marrow cells in supplemented RPMI 1640 for 7 days in the presence of 10% supernatant from the M-CSF producing fibroblast cell line L929. After removal of nonadherent cells, macrophages were detached using 5mM EDTA treatment, washed, counted, and replated at a density of 10x6 cells/mL. Macrophages were cultured for 24 hours in medium (control) or in the presence of IL-4 (10mg/mL). F4/80 positive macrophages were sorted with a purity of 99% and RNA was isolated (Trizol/Chloroform) according to the manufacturer's instructions. RNA was labeled (biotin), fragmented, and hybridized to the arrays according to the manufacturer's recommendations.
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| Contributor(s) |
Huber S, Voehringer D, Hoffmann R |
| Citation(s) |
20625006 |
| |
| Submission date |
Jan 25, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Silke Huber |
| E-mail(s) |
[email protected]
|
| Phone |
0049-89-2180-75653
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| Organization name |
LMU Munich
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| Department |
Institute for Immunology
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| Lab |
Dr. David Voehringer
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| Street address |
Goethestrasse 31
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| City |
München |
| ZIP/Postal code |
80336 |
| Country |
Germany |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA124115 |
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