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Series GSE20549 Query DataSets for GSE20549
Status Public on Feb 27, 2010
Title Expression data from H1299 and H460 lung cancer cell lines
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify a set of genes related to radioresistance, we analyzed the time-series gene expression profiles of radioresistant H1299 and radiosensitive H460 lung cancer cells in response to 2 Gy of ionizing radiation (IR) by performing quadratic regression (QR) analysis. Out of the 21,331 genes, we selected 6,538 genes by QR analysis from the gene expression profile of H460 cells and 6,086 genes from that of H1299 cells. Most of the genes identified in the H460 cells were classified into continuously up- or down-regulated groups, while the major QR groups were transiently changed groups in the H1299 cell line. From gene ontology analysis of the major QR groups, the DNA damage response was commonly enriched in both cell lines. DNA repair-related genes such as ATM, ATR, TP53BP1, BRCA1, MRE11, NBN and RAD50 were particularly up-regulated in H1299 cells. Suppression of these DNA repair-related genes using siRNA made H1299 cells radiosensitive to ionizing radiation. The data suggest that differential responses to DNA damage confer radioresistance to cancer cells, and provide potential novel targets for sensitizing radiotherapy.
 
Overall design We analyzed the time-series gene expression profiles of radioresistant H1299 and radiosensitive H460 lung cancer cells in response to 2 Gy of ionizing radiation (IR) by performing quadratic regression (QR) analysis to identify genes associated with radioresistance
 
Contributor(s) Park W
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Submission date Feb 26, 2010
Last update date Jul 26, 2018
Contact name Woong Yang Park
E-mail(s) [email protected]
Phone +82-02-740-8241
Fax +82-02-744-4534
Organization name Seoul National University
Department Biochemistry and Molecular Biology
Lab Molecular and Genomic Medicine
Street address 28 Yongondong, Chongnogu
City Seoul
ZIP/Postal code 110-799
Country South Korea
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (42)
GSM516315 H460 at T0, biological rep1
GSM516316 H460 at T0, biological rep2
GSM516317 H460 at T0, biological rep3
Relations
BioProject PRJNA125307

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Supplementary file Size Download File type/resource
GSE20549_RAW.tar 171.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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