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| Status |
Public on Aug 08, 2023 |
| Title |
Determination of WatR binding site in Methylomonas sp. DH-1 after lactate adaptive evolution |
| Organism |
Methylomonas sp. DH-1 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
JHM80 strain was obtained by adaptive evolution of Methylomonas sp. DH-1 strain against 8.0 g/L of lactate. The tolerance against lactate of JHM80 was obtained by the overexpression of watR (weak acid tolerance regulator). In order to discover the target genes of WatR, FLAG epitope tag was added to WatR of JHM80 strain and ChIP-seq was performed.
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| Overall design |
In order to discover the target genes and binding consensus of WatR, ChIP-seq was performed in the normal growth condition.
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| Contributor(s) |
Cha S, Cho YJ, Hahn JS |
| Citation(s) |
37464261 |
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| Submission date |
Jun 15, 2022 |
| Last update date |
Aug 09, 2023 |
| Contact name |
Yong-Joon Cho |
| Organization name |
Seoul National University
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| Street address |
1 Gwanak-ro, Gwanak-gu
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| City |
Seoul |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
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| Platforms (1) |
| GPL32357 |
NextSeq 550 (Methylomonas sp. DH-1) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE206217 |
Regulation of acetate tolerance by unannotated small open reading frame (smORF) polypeptides in Methylomonas sp. DH-1 |
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| Relations |
| BioProject |
PRJNA849631 |
