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| Status |
Public on Jun 21, 2010 |
| Title |
mRNA immunoprecipitated with CUGBP1 in C2C12 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dramatic changes in gene expression occur in response to extracellular stimuli and during differentiation. Although transcriptional effects are important, alterations in mRNA decay also play a major role in achieving rapid and massive changes in mRNA abundance. Moreover, just as transcription factor activity varies between different cell types, the factors influencing mRNA decay are also cell-type specific.GREs are recognized by CUGBP1, an RNA-binding protein and instability factor whose function is affected in several neuromuscular diseases. To dectect the mRNA associated with CUGBP1, we utilized RNA immunoprecipitation followed by microarray (RIP-Chip) to identify CUGBP1-associated transcripts.
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| Overall design |
To identify the mRNA associated with CUGBP1, we performed the immunoprecipitation followed by microarray (RIP-Chip)
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| Contributor(s) |
Lee JE, Lee JY, Wilusz J, Tian B, Wilusz CJ |
| Citation(s) |
20574513 |
| |
| Submission date |
Apr 07, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Ju Youn Lee |
| Organization name |
UMDNJ-New Jersey Medical School
|
| Department |
Biochemistry
|
| Street address |
185 South Orange Ave. MSB-E501
|
| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07101 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE21236 |
Systematic analysis of cis-elements in unstable mRNAs demonstrates that CUGBP1 is a key regulator of mRNA decay in muscle cells |
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| Relations |
| BioProject |
PRJNA129713 |
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