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| Status |
Public on Apr 08, 2010 |
| Title |
Differential gene expression in mouse retina related to regional differences in vulnerability to hyperoxia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Purpose: In the C57BL/6J mouse retina, hyperoxia-induced degeneration of photoreceptors shows strong regional variation, beginning at a locus ~0.5mm inferior to the optic disc. To identify gene expression differences that might underlie this variability in vulnerability, we have used microarray techniques to describe regional (superior-inferior) variations in gene expression in the retina.
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| Overall design |
Methods: Young adult C57BL/6J mice raised in dim cyclic illumination (12h 5lux, 12h darkness) were exposed to hyperoxia (75% oxygen for 2w). Retinas were collected from hyperoxia-exposed and control animals without fixation, and divided into superior and inferior halves. RNA was extracted from each sample, purified and hybridised to mouse 1.0 ST arrays (Affymetrix). The consistency of the microarray results was assessed with quantitative PCR for selected genes. Expression data were analysed to identify genes and ncRNAs whose differential expression between superior and inferior retina could be associated with relative vulnerability to hyperoxia.
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| Contributor(s) |
Zhu Y, Peng K, Ohms S |
| Citation(s) |
20454693 |
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| Submission date |
Apr 07, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Yuan Zhu |
| E-mail(s) |
[email protected]
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| Phone |
+61 2 61254489
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| Organization name |
Australian National University
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| Department |
Research School of Biology
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| Street address |
Bldg 46, RSBS, ANU
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| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA126521 |
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