Expression profiling by high throughput sequencing
Summary
We explored the effects of nociceptors on DC under various conditions by means of in vitro coculture of Flt3L-induced bone marrow-derived DCs and primary mouse nociceptors.
Overall design
Day 9 Flt3L-induced bone marrow DCs were cultured alone or with nociceptors and various stimuli overnight. DC subsets were FACS-sorted and sequenced