|
| Status |
Public on Dec 01, 2024 |
| Title |
Single-cell transcriptomic analysis reveals transcriptional and cell subpopulation differences between human and pig immune cells |
| Organisms |
Homo sapiens; Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The pig is a promising candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xeno-transplant rejection and hematopoietic reconstitution. We performed single-cell RNA-seq on human and pig immune cells. High oxidative phosphorylation, HIF-1, glycolysis, and lysosome-related gene expressions in pig CD14+ monocytes were observed, whereas pig CD14+ monocytes exhibited lower levels of cytokine receptors and JAK-STAT-related genes. Pig activated CD4+T cells decreased cell adhesion and inflammation, while enriched for migration and activation processes. Porcine GNLY+CD8+T cells reduced cytotoxicity and increased proliferation compared with human GNLY+CD8+T cells. Pig CD2+CD8+γδT cells were functionally homologous to human CD2+CD4+ γδT cells. Pig CD2-CD8-γδT cells expressed genes with quiescent and precursor characteristics, while CD2-CD8+γδT cells expressed migration and memory-related molecules. Pig CD24+ and CD5+B cells are associated with inflammatory responses. Our research with integrated scRNA-seq enables a deeper understanding of the development and function of porcine immune cells.
|
| |
|
| Overall design |
Pig blood was collected from the anterior vena cava, and human blood was collected from the median cubital vein. PBMCs were isolated by Ficoll gradient centrifugation.
|
| |
|
| Contributor(s) |
Zhang Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 07, 2022 |
| Last update date |
Dec 01, 2024 |
| Contact name |
Zhaoqi Zhang |
| E-mail(s) |
[email protected]
|
| Phone |
18310855988
|
| Organization name |
University of Chinese Academy of Sciences
|
| Street address |
Beijing China Institute of Zoology，Chinese Academy of Sciences
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100000 |
| Country |
China |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL20983 |
Illumina NextSeq 500 (Sus scrofa) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA909593 |
Less...
More...