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| Status |
Public on Feb 09, 2005 |
| Title |
Anti-prolif. effect of E2 in breast cancer cells that re-exp. ERalpha is mediated by aberr. regulat. of cell cycle genes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating them with 0ยท01% ethanol (vehicle control) or 10-8 M 17beta-estradiol (E2). After 48 h of treatment, total RNA was isolated and used for transcript profiling on Affymetrix GeneChips. Three independent biological replicates of this experiment were carried out.
Keywords: ordered
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| Contributor(s) |
Moggs Jg, Murphy Tc, Lim F, Moore Dj, Stuckey R, Antrobus K, Kimber I, Orphanides G |
| Citation(s) |
15821115 |
| |
| Submission date |
Feb 07, 2005 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Fei Ling Lim |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)1234222450
|
| Organization name |
Unilever
|
| Street address |
Colworth Science Park
|
| City |
Sharnbrook |
| State/province |
Bedfordshire |
| ZIP/Postal code |
MK44 1LQ |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA91353 |
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