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| Status |
Public on May 03, 2023 |
| Title |
Highly-conserved regulatory activity of the ANR family in the virulence of diarrheagenic bacteria through interaction with Master and Global regulators |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To gain mechanistic insights into this phenomenon, we characterized the regulatory role of Rnr in the virulence of prototype EPEC strain E2348-69 by genetic, biochemical, and human organoid-based approaches. Accordingly, RNA-seq analysis revealed more than 500 genes differentially regulated by Rnr, including the type-3 secretion system (T3SS).
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| |
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| Overall design |
RNA was extracted from EPEC E2348/69 derivatives (WT EPEC, EPECrnr, and EPECrnr(pRnr) grown in DMEM-HG
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| |
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| Citation(s) |
37120613 |
| |
| Submission date |
Feb 20, 2023 |
| Last update date |
May 03, 2023 |
| Contact name |
Araceli E Santiago |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Virginia
|
| Department |
Pediatrics
|
| Lab |
CHRC
|
| Street address |
409 Lane Road
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL25368 |
Illumina NovaSeq 6000 (Escherichia coli) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA936843 |
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