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| Status |
Public on Dec 06, 2014 |
| Title |
VEGF induction of HUVEC and CEP |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Angiogenesis, the formation of new capillaries by sprouting from preexisting vessels, is mainly induced by VEGF-A. To identify genes which are induced by VEGF-A in endothelial cells and genes which are differently expressed or VEGF-A induced in HUVEC and CEP, HUVEC and CEP were starved and induced by VEGF-A165 for 30, 60 and 150min. RNA of induced and uninduced cells was isolated and subjected to microarray analysis using Affymetrix microarray.
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| Overall design |
Human umbilical vein endothelial cells and circulating endothelial progenitor cells were grown in complete EGM-2 medium in dense culture for four days without change of medium and before the stimulation in EGM-2 medium without growth factors over night. The cells were stimulated with 100ng/ml VEGF-A165 for 30, 60 and 150 min. CD34 positive cells were isolated by MACS from cord blood. RNA was isolated with Trizol according to the manufacturer's instructions.
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| Contributor(s) |
Testori J, Bilban M, Hofer E |
| Citation missing |
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| |
| Submission date |
Jul 05, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Julia Testori |
| Organization name |
Medical University Vienna
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| Department |
Vascular Biology and Thrombosis Research
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| Street address |
Lazarettgasse 19
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| City |
Wien |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA128305 |
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