|
| Status |
Public on Dec 06, 2014 |
| Title |
CEP_VEGF_150min |
| Sample type |
RNA |
| |
|
| Source name |
CEP, starved, 150 min VEGF-A induced
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Circulating endothelial progenitor cells, isolated from cord blood
treatment: VEGF
time: 150 min
|
| Treatment protocol |
Endothelial cells were induced for 150min with 100ng/ml VEGF-A.
|
| Growth protocol |
Endothelial cells were kept in complete EGM-2 Medium in dense culture without change of medium and before the stimulation in EGM-2 medium without growth factors over night.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Description |
Gene expression data
|
| Data processing |
The data were analyzed using RMA (Robust multiarray average) Signal extraction and normalization.
|
| |
|
| Submission date |
Jul 05, 2010 |
| Last update date |
Dec 06, 2014 |
| Contact name |
Julia Testori |
| Organization name |
Medical University Vienna
|
| Department |
Vascular Biology and Thrombosis Research
|
| Street address |
Lazarettgasse 19
|
| City |
Wien |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE22695 |
VEGF induction of HUVEC and CEP |
|
