NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE227760 Query DataSets for GSE227760
Status Public on May 09, 2023
Title Context dependent function of transcriptional regulator Rap1 in gene silencing and activation [ChIP-seq III]
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary As part of a study on the mechanism of Sir-based silencing at HML in S.cerevisiae, and the contribution of Rap1 to transcription of the locus, we performed ChIP-seq of multiple components of RNA Pol II machinery in different genetic backgrounds including: sir4∆, SIR+, and with or without a mutation that abbrogated Rap1 binding at the alpha promoter.
 
Overall design Taf1-3xFLAG ChIPseq; Sua7-3xFLAG ChIPseq; Elf1-3xFLAG ChIPseq; Rpb3-3xFLAG ChIPseq in silenced (SIR) or unsilenced (sir4∆) cells, with or without a mutation in the alpha2 promoter.
 
Contributor(s) Bondra ER, Rine JD
Citation(s) 37214837, 37769255
Submission date Mar 20, 2023
Last update date Oct 18, 2023
Contact name Eliana Rose Bondra
Organization name University of California, Berkeley
Department Molecular and Cell Biology
Lab Rine
Street address 440 Barker Hall
City Berkeley
State/province California
ZIP/Postal code 94720
Country USA
 
Platforms (2)
GPL22715 Illumina MiniSeq (Saccharomyces cerevisiae)
GPL27812 Illumina NovaSeq 6000 (Saccharomyces cerevisiae)
Samples (76)
GSM7107664 SIR_wtpromoter_Taf1FLAG ChIP rep1
GSM7107665 SIR_wtpromoter_Taf1FLAG input rep1
GSM7107666 SIR_wtpromoter_Taf1FLAG ChIP rep2
This SubSeries is part of SuperSeries:
GSE227763 Context dependent function of transcriptional regulator Rap1 in gene silencing and activation
Relations
BioProject PRJNA946783

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE227760_RAW.tar 4.9 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap