|
| Status |
Public on May 09, 2023 |
| Title |
sir4∆_mutpromoter_Taf1FLAG ChIP rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Saccharomyces cerevisiae
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
tissue: Saccharomyces cerevisiae
cell line: JRy15444
genotype: , hml@2delta::CRE, ura3delta::pGPD:loxP:yEmRFP;tCYC1:hygMX:loxP:yEGFP:tADH1; 3xV5_Rap1-N-terminal; sir4delta::k.l.URA3; matdelta::k.l.LEU2
treatment: anti-FLAG® M2 antibody (MilliporeSigma, product F1804) and DynaBeads Protein G magnetic beads (ThermoFisher Scientific catalog number 10003D)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were lysed by mechanical disruption in a bead beater. Chromatin was fragmented by sonication. IPs were performed using 5mL mouse monoclonal anti-FLAG® M2 antibody (MilliporeSigma, product no F1804) and 50uL DynaBeads Protein G magnetic beads (ThermoFisher Scientific catalog number 10003D). DNA was purified using Qiagen spin column kit
NEB Ultra II library prep kit
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina MiniSeq |
| |
|
| Data processing |
FASTQ generation using Illumina Casava v2.2.
Raw reads were mapped to SacCer3 genome modified to include the mutant promoter site where applicable, and mat∆hmr∆ or hml∆mat∆, using Bowtie2 with following options: --local --soft-clipped-unmapped-tlen --no-unal --no-mixed --no-discordant
PCR duplicates were removed using, sequentially, SAMtools -fixmate -sort -markdup
bedgraph files were generated that included reads between 0-500bp (average fragment size from sonication) and normalized to the genome-wide median (median excluded subtelomeres, chromosome III, and rDNA)
Assembly: SacCer3 (modified)
Supplementary files format and content: bedgraph (normalized to genome-wide median)
|
| |
|
| Submission date |
Mar 20, 2023 |
| Last update date |
May 09, 2023 |
| Contact name |
Eliana Rose Bondra |
| Organization name |
University of California, Berkeley
|
| Department |
Molecular and Cell Biology
|
| Lab |
Rine
|
| Street address |
440 Barker Hall
|
| City |
Berkeley |
| State/province |
California |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platform ID |
GPL22715 |
| Series (2) |
| GSE227760 |
Context dependent function of transcriptional regulator Rap1 in gene silencing and activation [ChIP-seq III] |
| GSE227763 |
Context dependent function of transcriptional regulator Rap1 in gene silencing and activation |
|
| Relations |
| BioSample |
SAMN33832057 |
| SRA |
SRX19733265 |
