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| Status |
Public on Oct 15, 2010 |
| Title |
Sciatic nerve FAIRE-chip and ChIP-Chip with Egr2/Krox20 and Sox10 |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Egr2/Krox20 and Sox10 regulate genes involved in formation of myelination in the peripheral nervous system. ChIP-chip assays were performed on rat sciatic nerve at P15, a peak timepoint of myelination. In addition, Faire was used to identify areas of open chromatin.
This experiment includes a custom ChIP-chip design incorporating many genes that are dynamically regulated during peripheral nerve myelination. Two antibodies were used for Egr2, Abcam and Covance PRB-236P.
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| |
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| Overall design |
Egr2 and Sox10 ChIP samples were hybridized along with total input. In addition, FAIRE samples were hybridized relative to input DNA
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| |
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| Contributor(s) |
Svaren J, Srinivasan R, Jones EA |
| Citation(s) |
21044070, 21653862 |
| |
| Submission date |
Aug 16, 2010 |
| Last update date |
Mar 30, 2012 |
| Contact name |
John Svaren |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Wisconsin
|
| Department |
Comparative Biosciences
|
| Lab |
Waisman Center
|
| Street address |
1500 Highland Ave.
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL10812 |
UW_NimbleGen_Rat_321K_Array design 081105 |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA130963 |
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