|
| Status |
Public on May 19, 2011 |
| Title |
Expression data from Rad21 knock-down in ES cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The Cohesin complex has recently been described to regulate gene expression. We wanted to determine the gene expression profile specific in mouse ES cells after depletion of the Cohesin subunit Rad21.
We used microarrays to detail the global programme of gene expression underlying depletion of Rad21 and identified distinct early development related genes up-regulated and many pluripotency related genes downregulated.
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| |
|
| Overall design |
Rad21 was depleted in R1/E ES cells for 48h using esiRNAs against Rad21. An esiRNA against non-targeting Luciferase was used as a negative control
|
| |
|
| Contributor(s) |
Nitzsche A, Paszkowski-Rogacz M |
| Citation(s) |
21589869 |
| |
| Submission date |
Sep 01, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Maciej Paszkowski-Rogacz |
| Organization name |
Technische Universität Dresden
|
| Department |
UCC / Medical Faculty and University Hospital Carl Gustav Carus
|
| Lab |
Medical Systems Biology (Prof. Buchholz)
|
| Street address |
Tatzberg 47/49
|
| City |
Dresden |
| ZIP/Postal code |
01307 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (8)
|
| GSM589801 |
Luc control esiRNA, biological rep1 |
| GSM589802 |
Luc control esiRNA, biological rep2 |
| GSM589803 |
Luc control esiRNA, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE24030 |
The Cohesin Complex Cooperates with Pluripotency Transcription Factors in the Maintenance of Embryonic Stem Cell Identity |
|
| Relations |
| BioProject |
PRJNA133179 |
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