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| Status |
Public on Sep 15, 2023 |
| Title |
Nanostring of gene expression in intrahepatic leukocytes from mouse fed a chow or high-fat, -fructose, and -cholesterol (FFC) diet and treated with vehicle or a pharmacological inhibitor of the receptor for advanced glycation end products (RAGE), TTP488 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Intrahepatic macrophages in nonalcoholic steatohepatitis (NASH) are heterogenous and include proinflammatory recruited monocyte derived macrophages. RAGE is expressed on macrophages and can be activated by damage associated molecular patterns (DAMPs) upregulated in NASH, yet the role of macrophage-specific RAGE signaling in NASH is unclear. Therefore, we hypothesized that a subset of RAGE expressing macrophages are proinflammatory and mediate liver inflammation in NASH. We profiled the transcriptome of intrahepatic leukocytes in a murine model of diet induced NASH where mice received vehicle or a pharmacological RAGE inhibitor (TTP488). By Nanostring nCounter Immunology Codeset Analysis of IHLs, we observed that RAGE inhibition resulted in amelioration of FFC diet induced activation of macrophage and T cell pathways which was associated with attenuation of liver inflammation.
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| Overall design |
C57BL/6J mice were fed a high-fat, -fructose, and -cholesterol (FFC) diet to induce NASH or chow diet as control for 5 months. FFC fed mice received daily intraperitoneal injections of an antagonist of the extracellular domain of RAGE (TTP488) or vehicle for 30 days. After enzymatic digestion of equal amounts of liver tissue within each dietary group, IHLs were isolated by density Percoll-gradient ultracentrifugation. Gene expression analysis was conducted using nCounter Mouse Immunology Codeset (Nanostring, Seattle, WA) on IHLs from 3 biological replicates within each group.
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| Contributor(s) |
Parthasarathy G, Malhi H |
| Citation(s) |
38175729 |
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| Submission date |
Sep 14, 2023 |
| Last update date |
Apr 05, 2024 |
| Contact name |
Harmeet Malhi |
| E-mail(s) |
[email protected]
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| Organization name |
Mayo Clinic
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| Street address |
200 1st ST SW
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| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (1) |
| GPL19964 |
nCounter Mouse Immunology Panel |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE243295 |
Nanostring of gene expression in intrahepatic leukocytes from mice fed a high-fat, -fructose, and -cholesterol (FFC) diet |
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| Relations |
| BioProject |
PRJNA1017609 |
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