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Series GSE244788 Query DataSets for GSE244788
Status Public on Aug 01, 2024
Title Leo1 and Set1-mediated H3K4me3 contribute to sterol homeostasis in yeast [RNA-seq]
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by high throughput sequencing
Summary Project Abstract : Trimethylation of histone H3 lysine 4 (H3K4me3) is predominantly associated with transcriptional start sites (TSSs) and is believed to facilitate transcription initiation. Furthermore, H3K4me3 plays a role in defining cell fate and specific cellular functions. Nevertheless, the precise function of H3K4me3 in transcription activation remains a topic of ongoing debate. The Polymerase-associated factor 1 complex (Paf1C), which is integral to various transcription-related cellular processes, consists of five highly conserved subunits: Paf1, Ctr9, Rtf1, Cdc73, and Leo1. While all subunits of Paf1C are indispensable for the maintenance of H3K4 methylation levels, it is noteworthy that strains lacking Leo1 exhibited unaltered levels of H3K4me3. To elucidate the role of H3K4me3, we conducted a transcriptome analysis coupled with ChIP-sequencing of H3K4me3 in cells lacking Leo1. Our research uncovers a distinctive role of Leo1 in yeast, whereby it plays a pivotal role in maintaining sterol homeostasis through the suppression of Upc2 expression. Importantly, this role stands apart from the functions of other Paf1C subunits. H3K4me3 is essential for promoting the expression of sterol uptake genes that are Upc2-dependent when Leo1 is absent. Additionally, Set1 contributes to sterol homeostasis by regulating iron metabolism and mitochondrial functions rather than directly suppressing Upc2 expression. Therefore, our findings reveal a novel role for Leo1 in sterol homeostasis and highlight the importance of H3K4me3 in promoting transcription of response genes required for sterol uptake.
 
Overall design Sample number (1-6): We performed RNA-seq with WT(1,2), Δleo1(3,4), and Δset1(5.6) strains. Experiments were performed in duplicates.
 
Contributor(s) Lee J, Park S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Oct 06, 2023
Last update date Aug 01, 2024
Contact name Shinae Park
E-mail(s) [email protected]
Organization name Kangwon National University
Street address Kangwondaehak-gil
City Chuncheon-si
ZIP/Postal code KS007
Country South Korea
 
Platforms (1)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
Samples (6)
GSM7829719 1_BY4741-1
GSM7829720 2_BY4741-2
GSM7829721 3_dleo1-1
This SubSeries is part of SuperSeries:
GSE244789 Leo1 and Set1-mediated H3K4me3 contribute to sterol homeostasis in yeast
Relations
BioProject PRJNA1024899

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE244788_RAW.tar 182.5 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA

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