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| Status |
Public on Jul 22, 2011 |
| Title |
Genome-wide analysis of the effect of cryopreservation on peripheral blood mononuclear cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of cryopreservation effects on peripheral blood mononuclear cells at gene expression level. The hypothesis tested in the present study was that cryopreservation has an influence on the transcriptome profile of peripheral blood mononuclear cells. Results indicated remarkable changes in expression patterns upon cryopreservation of PBMCs, with a strong loss of signal intensities to background levels for several transcripts.
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| Overall design |
Total RNA obtained from isolated peripheral blood mononuclear cells of healthy human subjects eihter cryopreserved in liqiud nitrogen (frozen) or direclty lysed in Trizol after isolation (fresh).
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| Contributor(s) |
Debey-Pascher S, Staratschek-Jox A, Schultze J |
| Citation(s) |
21704280 |
| |
| Submission date |
Oct 18, 2010 |
| Last update date |
Feb 15, 2013 |
| Contact name |
Joachim L Schultze |
| E-mail(s) |
[email protected]
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| Organization name |
University of Bonn
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| Department |
LIMES Institute
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| Lab |
Genomics & Immunoregulation
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| Street address |
Carl-Troll-Str. 31
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| City |
Bonn |
| ZIP/Postal code |
53115 |
| Country |
Germany |
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| Platforms (1) |
| GPL6102 |
Illumina human-6 v2.0 expression beadchip |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE24758 |
Cryopreservation effects on peripheral blood |
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| Relations |
| BioProject |
PRJNA133391 |
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