|
| Status |
Public on Jul 22, 2011 |
| Title |
frozen PBMC_18 |
| Sample type |
RNA |
| |
|
| Source name |
frozen peripheral blood mononuclear cells
|
| Organism |
Homo sapiens |
| Characteristics |
age: 54 years
cell type: PBMC
cell source: healthy subject
protocol: frozen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy MiniElute Cleanup Kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with denaturing gel electrophoresis and UV measurement.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina© TotalPrep™ RNA Amplification Kit (Applied Biosystems, Darmstadt, Germany).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate frozen
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Oct 18, 2010 |
| Last update date |
Dec 21, 2011 |
| Contact name |
Joachim L Schultze |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Bonn
|
| Department |
LIMES Institute
|
| Lab |
Genomics & Immunoregulation
|
| Street address |
Carl-Troll-Str. 31
|
| City |
Bonn |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6102 |
| Series (2) |
| GSE24753 |
Genome-wide analysis of the effect of cryopreservation on peripheral blood mononuclear cells |
| GSE24758 |
Cryopreservation effects on peripheral blood |
|
