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| Status |
Public on Oct 28, 2010 |
| Title |
Expression profiles in WT and MLL1-KO MEF at two different circadian time point |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We found that a H3K4 specific histone methyltransferase MLL1, a mammalian homologue of Drosophila trithorax, is essential for circadian transcription. MLL1 is in a complex with CLOCK:BMAL1 and contributes to their rhythmic recruitment to circadian promoters and cyclic H3K4 tri-metylation. To analyze the function of MLL1 on circadian gene regulation, we performed comparative microarray analysis of global gene expression levels in WT and MLL1-deficient MEF, at two different circadian time points (CT18 and CT30). This analysis identified several genes whose expression levels were remarkably changed between CT18 and CT30 in WT and MLL1-KO MEF. Typical clock-regulated genes such as Per2, Per3, Bmal1, or Dbp were found to be changing in WT but not in MLL1-KO MEFs.
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| |
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| Overall design |
Wild-type and MLL1-KO MEF at two circadian time point (CT18 and 30), three replicates for each hour.
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| |
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| Contributor(s) |
Katada S, Sassone-Corsi P |
| Citation(s) |
21113167 |
| |
| Submission date |
Oct 27, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Sayako Katada |
| E-mail(s) |
[email protected]
|
| Organization name |
UC Irvine
|
| Street address |
Rm2226B GNRB Dep. Pharmacology UC Irvine
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92697 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA132007 |
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