|
| Status |
Public on Nov 23, 2010 |
| Title |
KLF13 regulate memory-like CD8 T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
“Memory-like T cells” are a subset of thymic cells that acquire effector function through the maturation process rather than interaction with specific antigen. Disruption of genes encoding T cell signaling proteins or transcription factors have provided insights into the differentiation of such cells. We show here that in BALB/c but not C57BL/6 mice, a large portion of thymic CD4-CD8+ T cells exhibit a memory-like phenotype. In BALB/c mice, IL-4 secreted by invariant natural killer T (iNKT) cells is both essential and sufficient for the generation of memory-like T cells. In C57BL/6 mice, iNKT cells are less abundant, producing IL-4 that is insufficient to induce thymic memory-like CD8+ T cells. BALB/c mice deficient in the transcription factor Kruppel-like factor (KLF) 13 have comparable numbers of iNKT cells to C57BL/6 mice and extremely low levels of thymic memory-like CD8+ T cells. This work documents the dramatic impact of a small number of KLF13-dependent iNKT cells.
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| |
|
| Overall design |
FACS-sorted naive splenic CD8 T cells from wild type and KLF13 ko mice were compared.
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| |
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| Contributor(s) |
Lai D, Clayberger C, Krensky A |
| Citation(s) |
21482696 |
| |
| Submission date |
Nov 19, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Dazhi Lai |
| Organization name |
NIH
|
| Department |
NHLBI
|
| Lab |
LMI
|
| Street address |
10 Center Dr, 7B20A
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA134641 |
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