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| Status |
Public on Dec 07, 2010 |
| Title |
Expression data from IL-10+ and IL-10- CD8 T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
IL-10 is an anti-inflammatory cytokine that has been shown to be produced by antigen-specific CD8 T cells at the peak of viral encephalitis. We found that IL-10+CD8 T cells are more activated and cytolytic than IL-10-CD8 T cells.
We used microarrays to detect gene expression changes in directly ex vivo sorted CNS IL-10+ and IL-10- CD8 T cells from a neurotropic J2.2-V-1-infected mouse.
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| Overall design |
C57Bl/6 Vert-X (IL-10-eGFP) mice were infected intracranially with 500 PFU J2.2-V-1 virus and seven days later their brain lymphocytes from three mice were pooled and then isolated and negatively selected for CD8 T cells. The CD8 pooled brain lymphocytes were then sorted for IL-10+ and IL-10- CD8 T lymphocytes using GFP as a marker from the same samples. This was performed three times to obtain six samples total. RNA was extracted and hybridized to GeneChip Mouse GENE 1.0 ST arrays (Affymetrix).
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| Contributor(s) |
Perlman S, Trandem K |
| Citation(s) |
21317392 |
| |
| Submission date |
Dec 03, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Stanley Perlman |
| E-mail(s) |
[email protected]
|
| Phone |
3193358549
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| Organization name |
University of Iowa
|
| Department |
Department of Microbiology
|
| Street address |
51 Newton Road
|
| City |
Iowa City |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM634838 |
Brain CD8 T cells that are IL-10+ rep1 |
| GSM634839 |
Brain CD8 T cells that are IL-10- rep1 |
| GSM634840 |
Brain CD8 T cells that are IL-10+ rep2 |
| GSM634841 |
Brain CD8 T cells that are IL-10- rep2 |
| GSM634842 |
Brain CD8 T cells that are IL-10+ rep3 |
| GSM634843 |
Brain CD8 T cells that are IL-10- rep3 |
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| Relations |
| BioProject |
PRJNA135763 |
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