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| Status |
Public on Dec 02, 2024 |
| Title |
RNA-sea of Smad4 wild-type or Smad4 knockout KPC PDAC organoids from monocultures or co-cultures with pancreatic stellate cells and RNA-seq of flow-sorted PSCs from the same co-cultures. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-sequencing of flow-sorted Smad4 wild-type (WT) or Smad4 knockout (KO) KPC (i.e. KRASG12D; p53 mutant) pancreatic ductal adenocarcinoma (PDAC) organoids from monocultures or co-cultures with pancreatic stellate cells (PSCs) and RNA-sequencing of flow-sorted PSCs from the same co-cultures.
We investigated changes in transcriptome in Smad4 KO KPC PDAC organoids compared to Smad4 WT KPC PDAC organoids in monoculture or in co-culture with PSCs. We also investigated the changes in transcriptome in PSCs co-cultured with Smad4 WT or Smad4 KO KPC PDAC organoids.
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| Overall design |
44 samples in total. Comparative gene expression profiling analysis of RNA-seq data for flow-sorted Smad4 WT or Smad4 KO KPC PDAC organoids in monoculture or co-cultured with PSCs, and of co-cultured PSCs.
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| Contributor(s) |
Biffi G, Lloyd E, Jihad M |
| Citation missing |
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| |
| Submission date |
Apr 02, 2024 |
| Last update date |
Dec 02, 2024 |
| Contact name |
Giulia Biffi |
| E-mail(s) |
[email protected]
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| Organization name |
CRUK Cambridge Institute
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| Street address |
Robinson Way
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (44)
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| Relations |
| BioProject |
PRJNA1095551 |
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