|
| Status |
Public on Dec 02, 2024 |
| Title |
11_T6LOH_RosaSmad4KO_PSC21 |
| Sample type |
SRA |
| |
|
| Source name |
WT
|
| Organism |
Mus musculus |
| Characteristics |
cell line: WT
cell type: Organoid
treatment: Coculture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were collected in 1 mL of TRIzol Reagent and stored at -80C. The RNA was extracted using the the PureLink RNA mini kit (12183018A; Invitrogen)
Illumina mRNA library. mRNA library preparations were performed using 55 uL of 10 ng/mL per sample
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Estimated transcript counts were counted using Salmon (version 1.9.0)
Assembly: GRCm38_release-102_kmer23
Supplementary files format and content: Raw count data in tab-delimited salmon file
|
| |
|
| Submission date |
Apr 02, 2024 |
| Last update date |
Dec 02, 2024 |
| Contact name |
Giulia Biffi |
| E-mail(s) |
[email protected]
|
| Organization name |
CRUK Cambridge Institute
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE263080 |
RNA-sea of Smad4 wild-type or Smad4 knockout KPC PDAC organoids from monocultures or co-cultures with pancreatic stellate cells and RNA-seq of flow-sorted PSCs from the same co-cultures. |
|
| Relations |
| BioSample |
SAMN40730554 |
| SRA |
SRX24136363 |
