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| Status |
Public on Feb 19, 2011 |
| Title |
Bacillus subtilis CU1065: WT vs fur, fur vs fur fsrA, fur vs fur fbpAB, fur vs fur fbpC, and fur vs fur fbpABC |
| Organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by array
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| Summary |
Global tranascriptional profiling of Bacillus subtilis cells comparing fur mutant to mutants of the iron-sparing response: fur fsrA double mutant, fur fbpAB triple mutant, fur fbpC double mutant, and fur fbpABC quadruple mutant
Abstract of associated manuscript: The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated, in large part, by a small non-coding RNA named FsrA. FsrA functions together with three small, basic proteins (FbpABC) to repress many "low-priority" iron-containing enzymes. We have used transcriptome analyses to define the scope of the iron-sparing response and to define subsets of genes dependent on either FbpAB or FbpC for their repression. Enzymes of the tricarboxylic acid cycle, including both aconitase and succinate dehydrogenase, are major targets of FsrA-mediated repression and, as a consequence, flux through this pathway is significantly decreased under iron limitation. FsrA also mediates a physiologically significant repression of iron-sulfur containing enzymes required for ammonium assimilation (the GltAB glutamate synthase) and catabolism of lactic acid (LutABC). Repression of the lutABC operon requires both FsrA and FbpB which supports a model in which the Fbp proteins function to enable repression of subsets of the FsrA regulon.
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| Overall design |
WT vs fur (fur), fur vs fur fsrA (fsrA), fur vs fur fbpAB (AB), fur vs fur fbpC (C), and fur vs fur fbpABC (ABC). Each experiemnt (fur vs mutant) was conducted three times using three independent total RNA preparations (2 independent experiement + 1 independent dye swap).
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| Contributor(s) |
Smaldone GT, Helmann JD |
| Citation(s) |
22389480 |
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| Submission date |
Feb 18, 2011 |
| Last update date |
Feb 20, 2014 |
| Contact name |
John D. Helmann |
| E-mail(s) |
[email protected]
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| Phone |
607 255 6570
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| Organization name |
Cornell University
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| Department |
Microbiology
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| Street address |
372 Wing Hall
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
| GPL7420 |
Sigma Genosys Panorama Bacillus subtilis Gene Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA137241 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE27416_RAW.tar |
9.1 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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