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| Status |
Public on Dec 29, 2011 |
| Title |
CD69-dependent gene expression in activated CD4 T cells from the spleen of Mus musculus |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
CD69 is a transmembrane protein expressed on the surface of activated leukocyte. The ligand for CD69 and the intracellular signaling pathway of this molecule are yet unknown. It is widely used as a marker of activated lymphocyte, but its function in immune system is not known.
We used micro-array to define genes whose expression is regulated by activation antigene CD69.
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| Overall design |
CD4 T cells were isolated from the spleen of wt B6 and CD69-deficient B6 mice and in vitro activated with anti-CD3/anti-CD28 coated beads. On one groupe of wt B6 cells, CD69 was activated using a anti-CD69 and secoundary antibody. RNA extraction and hybridization on Affymetrix microarrays was performed for wt B6, CD69-activated wt B6 and CD69-deficient B6 CD4 T cells.
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| Contributor(s) |
Niess JH, Radulovic K, Holzmann K |
| Citation(s) |
22250092 |
| |
| Submission date |
Mar 07, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Karlheinz Holzmann |
| E-mail(s) |
[email protected]
|
| Organization name |
Ulm University
|
| Department |
Medical Faculty
|
| Lab |
Genomics Core Facility
|
| Street address |
Helmholtzstreet 8/1
|
| City |
Ulm |
| ZIP/Postal code |
D-89081 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM686140 |
wt B6 CD4 T cells biological replicate 1 |
| GSM686141 |
wt B6 CD4 T cells biological replicate 2 |
| GSM686142 |
wt B6 CD4 T cells biological replicate 3 |
| GSM686143 |
CD69-/- B6 CD4 T cells biological replicate 1 |
| GSM686144 |
CD69-/- B6 CD4 T cells biological replicate 2 |
| GSM686145 |
CD69-/- B6 CD4 T cells biological replicate 3 |
| GSM686146 |
CD69-activated CD4 T cells biological replicate 1 |
| GSM686147 |
CD69-activated CD4 T cells biological replicate 2 |
| GSM686148 |
CD69-activated CD4 T cells biological replicate 3 |
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| Relations |
| BioProject |
PRJNA138127 |
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