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| Status |
Public on Nov 01, 2024 |
| Title |
Multi-omics analysis of molecular changes during early progression of precancerous lesion to lung adenocarcinoma in never-smokers |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
The prevalence of lung adenocarcinoma (LUAD) has increased sharply in East Asia. Early diagnosis leads to better survival rates, but this requires an improved understanding of the molecular changes during early tumorigenesis, particularly in non-smokers. We performed whole exome-sequencing and RNA-sequencing of samples from 94 East Asian patients with precancerous lesions (25 with atypical adenomatous hyperplasia [AAH]; 69 with adenocarcinoma in situ [AIS]) and 73 patients with early invasive lesions (minimally invasive adenocarcinoma [MIA]). Cellular analysis revealed that the activities of endothelial and stromal cells could be used to categorize tumors into molecular subtypes within pathology-defined types of lesions. These subtypes were linked with the advanced radiology-defined type of lesions and corresponded to immune cell infiltration throughout the early progression of LUAD. Characterization of these lesion types identified positively selected mutation patterns and suggested that angiogenesis of the late-stage AIS type potentially contributes to tissue invasion of the MIA type. Our findings offer a novel resource that may help to improve early diagnosis and patient prognosis, and also suggest possible approaches for early disease interception.
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| Overall design |
Spatial transcriptomics data were generated using the 10x Visium CytAssist Spatial Gene Expression for the FFPE platform (11 × 11 mm, Human, PN-1000522)
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| Contributor(s) |
Hsu C |
| Citation missing |
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| Submission date |
Sep 14, 2024 |
| Last update date |
Nov 02, 2024 |
| Contact name |
Chia-Lang Hsu |
| E-mail(s) |
[email protected]
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| Organization name |
National Taiwan University Hospital
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| Department |
Department of Medical Research
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| Street address |
No. 7, Zhongshan S. Rd.
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| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1161183 |
