|
| Status |
Public on Sep 14, 2012 |
| Title |
Identification of differentially regulated genes upon overexpression of HLX in wild-type hematopoietic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The goal was to study the role of Hlx in hematopoiesis.
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| Overall design |
Sorted Lin-Kit+Sca-1+ cells from wild-type FVB/nJ bone marrow were infected with control (pCAD-IRES-GFP) or Hlx lentivirus (pCAD-IRES-GFP-Hlx) and cultured for 2 days in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene. Subsequently, GFP+ cells were sorted by FACS and RNA was prepared. Three replicates of each, control vector transduced and HLX-transduced cells were used.
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| |
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| Contributor(s) |
Kawahara M, Will B, Barreyro L, Bartholdy B, Mitsiades CS, Steidl UG |
| Citation(s) |
22897850 |
| |
| Submission date |
Mar 14, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Boris Bartholdy |
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Cell Biology
|
| Street address |
1300 Morris Park Ave
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE27947 |
Identification of differentially regulated genes in hematopoietic stem cells and URE leukemia cell line |
|
| Relations |
| BioProject |
PRJNA141719 |
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