|
| Status |
Public on Sep 14, 2012 |
| Title |
Identification of differentially regulated genes upon shRNA-mediated knock-down of HLX in the URE leukemia cell line |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To study the role of Hlx in hematopoietic differentiation and tumorigenesis, URE cells were infected with short-hairpin-containing pSIH1-H1-copGFP lentiviral vector (System Biosciences, Mountain View, CA) containing either nucleotide sequences targeting luciferase (shControl) or HLX (shHLX). After 24hrs incubation in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene, cells were cultured in fresh medium for several days. Subsequently, GFP+ cells were sorted by FACS and RNA was prepared.
|
| |
|
| Overall design |
URE cells were infected with short-hairpin-containing pSIH1-H1-copGFP lentiviral vector (System Biosciences, Mountain View, CA) containing either nucleotide sequences targeting luciferase (shControl) or HLX (shHLX). After 24hrs incubation in Iscove’s modified Dulbecco’s medium (IMDM) containing FBS, mIL-3, mIL-6 and mSCF with lentiviral supernatants in the presence of 8ug/ml polybrene, cells were cultured in fresh medium for several days. Subsequently, GFP+ cells were sorted by FACS and RNA was prepared. Three replicates of each, shControl and shHLX-transduced cells were used. The goal was to study the role of Hlx in hematopoietic differentiation and tumorigenesis.
|
| |
|
| Contributor(s) |
Kawahara M, Will B, Barreyro L, Bartholdy B, Mitsiades CS, Steidl UG |
| Citation(s) |
22897850 |
| |
| Submission date |
Mar 14, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Boris Bartholdy |
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Cell Biology
|
| Street address |
1300 Morris Park Ave
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE27947 |
Identification of differentially regulated genes in hematopoietic stem cells and URE leukemia cell line |
|
| Relations |
| BioProject |
PRJNA141721 |
Less...
More...